Other
Part:BBa_K4891012:Design
Designed by: Kexin Fei Group: iGEM23_Guangzhou-MedX (2023-06-27)
poxB
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 651
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 651
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 651
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 651
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
These recombinant plasmids pBAD33-ldhA/adhE/poxB/pta and pTargetF-sgRNAldhA/adhE/poxB/pta serve as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively.
Source
ldhA (encoding lactate dehydrogenase), adhE (encoding alcohol dehydrogenase), poxB (encoding pyruvate dehydrogenase) and pta (encoding phosphate acetyltransferase) are obtained from the genome of E. coli MG1655.